Mammalian Two-Hybrid System Offered by Creative BioMart

Two-hybrid systems are extremely powerful methods for detecting protein-protein interactions in vivo. Of note, Creative BioMart Mammalian Two-Hybrid Assay allows rapid and convenient analysis of protein-protein interactions in transfected mammalian cells. 

This assay uses a SEAP secreted reporter to measure the interactions between the two proteins of interest, eliminating the need for cell lysis. Because this two-hybrid assay is performed in mammalian cells, proteins encoded by mammalian cDNAs are more likely to be in their native conformation; therefore, post-translational modifications and experimental results are more likely to represent biologically significant interactions.

Features
Fast, convenient analysis of protein-protein interactions in mammalian cells
SEAP secreted reporter eliminates the need for cell lysis
Biologically relevant results are more likely in a mammalian system

Applications
Confirm protein-protein interactions in mammalian cells
Map interacting domains

A noteworthy point of preference of the mammalian two-hybrid assay over yeast two-hybrid system is that protein-protein interactions are considered in mammalian cell lines. Accordingly, this assay empowers specialists to study communications between mammalian proteins that may not fold accurately in yeast or that require post-translational modification or external stimulation that is not present in yeast.

Experts at Creative BioMart could be glad to discuss the details of intended interaction studies with its valued customers and develop experimental methods and strategies tailored to its customers'requirements. Customers can contact at contact@creative-biomart.com for more information.

Source: http://www.creativebiomart.net/Mammalian-Two-Hybrid-System.htm