Assay Kits Supplier—Creative BioMart

Assay kits, used in life science research, drug discovery & development as well as environmental monitoring in a broad range of applications such as studying disease pathways, or finding potential drug candidates or evaluating biopharmaceutical production processes.

With years of experience and expertise, Creative BioMart now offers thousands of assay kits in a variety of formats, including enzyme activity, inhibitor screening, epigenitic, apoptosis, cell damage, cell proliferation and cytotoxicity, cell signing, metabolism and other detections that have been exhaustively tested for superior quality.

These assays are regarded quite useful tools for the measuring of enzyme activity, monitoring of signal pathway activity, detecting and quantifying of cellular processes, for example cell death, cell viability/proliferation, or metabolic reaction to study changes in gene expression and to screen small molecule inhibitors.

Assay kits in Creative BioMart are ready-to-use and provide all reagents in necessity, directions, plates and analysis protocols to help its customers to quickly get exact and reproducible outcomes. With Creative BioMart’s assay kits, customers can get profoundly sensitive, precise, and precise results over a wide dynamic range with quick and efficient processing.

Assay kits in Creative BioMart include but not limited to: activity kits, metabolism kits, epigenetic kits, cellular kits, signaling kits, detection kits, etc.

Visit http://www.creativebiomart.net/assay-kits.htm to know more.

Trying to Find Membrane Protein Service?

Membrane proteins, proteins that interact with biological membranes, accounts for nearly half of know drug targets. It is estimated that almost 20-30% of all genes in most genomes encode membrane proteins. Membrane proteins as receptors and ion channels are vital regulators of cellular function and all of them are of great pharmaceutical importance.

The statistics of a recent survey may illustrate this well. The survey was meant to figure out the proportion of the classes of drug target in current drug therapies. And the results are as follows: receptors accounts for nearly half of the human drug targets and acting as therapeutic targets for a wide of disease conditions like: cancer and cardiovascular; following are enzymes which cover almost 30%; the third place was taken by hormones & factors; and ion channels take 5% of it. That is to say membrane protein account over 50% of the human drug target. So the importance of it is obvious.

However, good things are always hard to get. So does membrane proteins. It is said that they are notoriously difficult to prepare in a pure, correctly-folded form in sufficient quantity for drug discovery.

Since its foundation, Creative Biomart has been working with pharmaceutical, biotech, clinical and academic customers and researcher, providing them with excellent membrane protein-related products and services that help promote the customers’ research. Now, they have customer membrane protein production, cell line development ad membrane preparation and lipid-based protein immoblizition services for their customers to choose. For more information about this, you can visit http://www.creativebiomart.net/Membrane-Protein-Services.htm.

About Creative BioMart

Creative BioMart provides quality recombinant proteins, lysate, chromatography and native protein to the research community of biology, clinical research, molecular diagnostics and biopharmaceutical drug development. We are offering more than 1,000 recombinant proteins, peptides and antibodies. All the products are rigorously tested to meet the most demanding research needs. At the same time, lowest prices in the industry are always guaranteed.

Contact Creative BioMart

Address: 45-1 Ramsey Road Shirley, NY 11967, USA

Tel: 1-631-559-9269

Email: info@creative-biomart.com

Website: www.creativebiomart.net

How Will Protein Expression Market Develop in the Future?

Protein expression, another name of which is protein production, has been widely used in the life sciences, medicine and biotechnology. Researchers in the field of molecular biology use a large number of proteins and enzymes which are produced by expression systems. Significant application of expression system can also be found in the production of human insulin to treat diabetes and to produce certain proteins in biotechnology and industry. Currently, they are mostly used to produce proteins that are screened for drug discovery purpose. Generally speaking, commonly used protein expression systems include those derived from bacteria, yeast, baculovirus and mammalian cells.

An agency focuses on analyzing kinds of market did a survey about the protein expression market recently. They studied the expression system, products, applications and end users. For the expression system part, they estimated that the mammalian part would grow at the highest CAGR in the following four years for the demand for therapeutic proteins has been increased. In the product part, the reagents were found to have the largest share of the global market, and the services part would grow at the highest CAGR in the following four years. For the part of application, the researchers studied industrial proteins, research applications and therapeutic proteins. Among them, therapeutic proteins cover the largest share of the global market and are estimated to grow at the highest CAGR in the following four years. In their report, academic research, contract organizations, pharmaceutical and biotechnological companies and others were included in the end-user part. According to their statistics, the pharmaceutical and biotechnological companies have the largest share of the global protein market, while the contract research organizations would grow at the highest CAGR in the following four years. The global market is expected to grow fast and it may reach $ 1,400 million at the end of 2018.

As a world leading biotech company, Creative Biomart is committed to provide easy-to-use protein expression systems for its valued customers. Up to now, it has provided thousands of protein expression service for customers worldwide. Currently, it has nineteen types of expression system for customers to choose, including Bacterial expression systems (E.coli / Bacillus), Yeast expression systems (P pastoris / S cerevisiae), mammalian expression systems (CHO /293), Cell-free in vitro Protein Expression, etc. To know more detailed information, you can visit http://www.creativebiomart.net/Protein-Expression-Services.htm.

Stable Cell Line Services in Creative BioMart

Supported by years of experience and advanced facilities, Creative BioMart provides stable cell line service to cover its customers’ needs in the aspects of protein production and assay development. Not only have we developed cell lines stable at expression level, but also established stable cell lines ready for assay development. Creative BioMart stable cell line platform guarantees over-expressing of gene of interest in any cell hosts.

Generally, stable cell line services in Creative BioMart include protein production grade stable cell lines; assay grade stable cell lines; gene edited knock-out, knock-in custom cell lines.

Creative BioMart service provides a recombinant stable pool or stable single clone generated from our lentiviral vector transduction service, yielding high transfection efficiency. Scientists at Creative BioMart ensure you successfully generate a recombinant stable cell pool or clone with any gene in any host cell. Creative BioMart also provides conventional gene transfer methods for stable cell line development. For knock-out and knock-in cell lines, Creative BioMart offers BioCRISPRTM custom genome editing service.

Choosing Creative BioMart for stable cell line services, you can benefit from:

Stability: Creative BioMart has developed a system that makes the cell line more stable than ever. We have found that even after 25 generations, their expression level is comparable to earlier generations.

High expression: We provide the best system to create a stable cell line with capability of expression high amount of intracellular or secreted of the targeted protein if you request to develop a cell line high in protein yield.

Fast: High productivity stable clone approximately in 12 weeks.

To know more details about stable cell line services in Creative BioMart, you can visit http://www.creativebiomart.net/Stable-Cell-Line-Services-For-Protein.htm or email contact@creative-biomart.com

About Creative BioMart
Creative BioMart provides quality recombinant proteins, lysate, chromatography and native proteins to the research community of biology, clinical research, molecular diagnostics and biopharmaceutical drug development. We are offering more than 1,000 recombinant proteins, peptides and antibodies. All the products are rigorously tested to meet the most demanding research needs. At the same time, lowest prices in the industry are always guaranteed.
Contact Creative BioMart
Address: 45-1 Ramsey Road Shirley, NY 11967, USA
Tel: 1-631-559-9269
Email: info@creative-biomart.com
Website: www.creativebiomart.net

To Know More about Two-hybrid System

The two-hybrid system is a genetic method that utilizes transcriptional activity as a measure of protein-protein interaction. It depends on the secluded way of site-specific transcriptional activators, which comprise of a DNA-restricting area and a transcriptional actuation space. The DNA-restricting space serves to focus on the activator to the promoter district of particular qualities, and the enactment area contacts different proteins of the transcriptional hardware to empower translation to happen. The two-cross breed framework depends on the perception that the two areas of the activator don't need to be covalently connected and can be united by the collaboration of any two communicating proteins.

The application of this system requires two hybrids to be constructed: a DNA-restricting area intertwined to one protein, X, and a translation initiation space combined to another protein, Y. Those two hybrids are expressed in a cell containing one ore more reporter genes. One the off chance that the X and Y proteins cooperate, they make a functional activator by bringing the activation domain into close proximity to the DNA-binding domain, that can be identified by articulation of the reporter gene. While the assay has been generally performed in yeast cells, it works correspondingly in mammalian cells. Co-expression of proteins for the determination and characterization of protein interactions is achieved with Yeast Two-Hybrid technology.

With its various features, two-hybrid system is regarded as a quite useful tool for the analysis of protein-protein interactions. For example, it is highly sensitive, and is capable of detecting interactions that can not be detected by other methods. 

Mostly regularly utilized versions of the two-hybrid system all involve DNA-binding domains that get from the yeast Ga14 protein or the E. Coli LexA protein. Transcriptional activation domains are commonly derived from the Ga14 protein or the herps simplex virus VP16 protein. Reporter genes include the E. Coli lacZ gene and selectable yeast genes, for example: HIS3 and LEU2. An expanding number of activation domain libraries are now achievable for proteins from a wide range of creatures or particular mammalian tissues.

As a leader specialized in protein interaction analysis, Creative BioMart has acquired long experience and expert knowledge in protein interaction study. Contact to know more about Two-hybrid System as well as other protein services including protein expression services provided by Creative BioMart.

Get the Best Protein Expression And Purification Services

Proteins, large biological molecules that consist one or more long chains of amino acid residues, are clinically conformed to be the most essential molecules that are needed to form body mass for living beings. They direct our body activities, organize our thoughts and defend us against infection, thus keeping us healthy. Since the key roles they play, there are a bunch of research field to further study the proteins. Protein expression, protein labeling, protein quantification, etc are all case in point.

Protein expression, or in other words, protein production occurs after the gene expression. The stages after DNA has been transcribed to messenger RNA (mRNA) are also included in the process. Protein expression can find a wide range of applications: in the life sciences, biotechnology and medicine. Protein expression systems are mostly used to produce certain proteins in biotechnology and industry. Recently, it finds its application in the production of certain proteins that are screened for drug discovery purpose.

Currently, according to Wikipedia, there are approximately two broad kinds of protein expression systems which are cell-based systems and cell-free systems. Cell -based systems include bacterial systems and eukaryotic systems. How to choose the appropriate system among these numerous systems? The turn around time, the cost and the quality of course should be taken into consideration. What’s more? Can the process be streamlined or can the yields be improved? Those factors can also be a judge. The experience of doing this, to some extent, can also add a point.

Creative Biomart, a world leading biotech company that specializes in the protein expression and purification services, has provided these services to thousands of customers all over the world. And customers come back for the good quality and fast turnaround time. Up to now, Creative Biomart has kinds of expression systems for customers to choose, like bacterial expression systems, yeast expression systems, mammalian expression systems, cell-free in vitro protein expression, to name a few. The turnaround time can be as little as 4 weeks. The quality is also guaranteed.

To know more about the protein expression systems provided by Creative Biomart, you can visit http://www.creativebiomart.net/Protein-Expression-Services.htm.

About Creative Biomart

Creative Biomart provides quality recombinant proteins, diagnostic antibodies and antigens, diagnostic enzymes and pharmaceutical enzymes to the research community of biology, clinical research, molecular diagnostics and biopharmaceutical drug development. We are offering more than 1,000 recombinant proteins, peptides and antibodies. All the products are rigorously tested to meet the most demanding research needs. At the same time, lowest prices in the industry are always guaranteed.

Contact Creative Biomart

Address: 45-16 Ramsey Road Shirley, NY 11967, USA

Tel: 1-631-559-9269

Email: info@creative-biomart.com

Website: www.creativebiomart.net

Creative BioMart Seminar—for Recombinant Proteins Development

Creative BioMart, a world leading biotech company specialized in the supply of quality proteins, recombinant proteins, GMP proteins, and custom protein services, etc., launched a seminar to discuss the development of a more comprehensive list of recombinant proteins. Paricipants inlcude Creative BioMart staff scientists and sale staff. Holding this seminar, Creative BioMart aims to expand its providing for recombinant protein products.

Recombinant protein production has become an essential tool for providing the necessary amounts of a protein of interest to either research or therapy by providing adequate amounts of proteins for studying protein structure and function. Moreover, scaled-up facilities produce recombinant vaccines, hormones, antibodies, growth factors, blood components, and enzymes.

Creative BioMart is a well recognized brand for over 50,000 recombinant proteins for diverse researches around the world, ranging from proteins, cytokines, enzymes, hormones, kinase, chemokines to others.

Linna Green, the chief marketing staff in Creative BioMart, said in the seminar that “We found out that our customers’ needs for recombinant proteins is increasing by the day. So we decided to develop and introduce more products of this kind with our current products quality assured. We must work harder for early results. Guaranteed by our advanced technologies and facilities, we do believe the day is now faraway to come.”

Through this seminar. Creative BioMart got further advance in the development of recombinant proteins though it has already stood out in reputation and product quality.

About Creative BioMart

Creative BioMart provides quality recombinant proteins, diagnostic antibodies and antigens, diagnostic enzymes and pharmaceutical enzymes to the research community of biology, clinical research, molecular diagnostics and biopharmaceutical drug development. We are offering more than 1,000 recombinant proteins, peptides and antibodies. All the products are rigorously tested to meet the most demanding research needs. At the same time, lowest prices in the industry are always guaranteed.

Contact Creative BioMart

Address: 45-16 Ramsey Road Shirley, NY 11967, USA

Tel: 1-631-559-9269

Email: info@creative-biomart.com

Website: www.creativebiomart.net

Yeast Expression System Service in Creative BioMart

Yeast systems have been a staple for producing large amounts of proteins for industrial and biopharmaceutical use for many years. It is the most economical eukaryotic expression system for both secretion and intracellular expression. And it’s ideally suitable for large-scale production of recombinant eukaryotic proteins.

Take advantage of Creative BioMart's proprietary YeastXceedTM Technology, selected stable and durable production strains that resemble mammalian system are employed for high-yield, high-productivity protein processing, thus dramatically reducing the associated costs and inherent time requirements.

With expertise in protein expression spanning both P. pastoris and S. cerevisiae expression systems, Creative BioMart is the right choice for protein production via fed-batch pichia processes. The company’s staff can move help move your project forward.

Engineering-enhanced protein expression technologies in Creative BioMart will enable a high success rate over 95% over various recombinant proteins. And the proprietary YeastXceedTM technology is pushing the yields even higher. Many secretory proteins that bacterial expression systems yield as insoluble or as inactive inclusion bodies can be produced successfully by the company’s yeast expression system using native or yeast secretion signals.

In the past years, we have identified DNA elements that are instrumental in increasing translation rates when included in recombinant protein expression vectors. These elements are functional across eukaryotic production platforms, including mammalian cells, yeast and most fungal systems. On the basis of these elements, the company developed proprietary HostOptimTM technology that has been confirmed for a wide variety of industry relevant platforms.

For more information about Yeast Expression System Service in Creative BioMart, please contact 1-631-559-9269.

Mammalian Two-Hybrid System Offered by Creative BioMart

Two-hybrid systems are extremely powerful methods for detecting protein-protein interactions in vivo. Of note, Creative BioMart Mammalian Two-Hybrid Assay allows rapid and convenient analysis of protein-protein interactions in transfected mammalian cells. 

This assay uses a SEAP secreted reporter to measure the interactions between the two proteins of interest, eliminating the need for cell lysis. Because this two-hybrid assay is performed in mammalian cells, proteins encoded by mammalian cDNAs are more likely to be in their native conformation; therefore, post-translational modifications and experimental results are more likely to represent biologically significant interactions.

Features
Fast, convenient analysis of protein-protein interactions in mammalian cells
SEAP secreted reporter eliminates the need for cell lysis
Biologically relevant results are more likely in a mammalian system

Applications
Confirm protein-protein interactions in mammalian cells
Map interacting domains

A noteworthy point of preference of the mammalian two-hybrid assay over yeast two-hybrid system is that protein-protein interactions are considered in mammalian cell lines. Accordingly, this assay empowers specialists to study communications between mammalian proteins that may not fold accurately in yeast or that require post-translational modification or external stimulation that is not present in yeast.

Experts at Creative BioMart could be glad to discuss the details of intended interaction studies with its valued customers and develop experimental methods and strategies tailored to its customers'requirements. Customers can contact at contact@creative-biomart.com for more information.

Source: http://www.creativebiomart.net/Mammalian-Two-Hybrid-System.htm

Industry News Roundup for February, 2016: Biotech

A novel self-enhanced electrochemiluminescence immunosensor based on hollow Ru-SiO2@PEI nanoparticles for NSE analysis

Poly (ethylenimine) (PEI) and Ru(bpy)32+-doped silica (Ru-SiO2) nanoparticles were simply mixed together to prepare a novel self-enhanced electrochemiluminescence (ECL) composite of Ru-SiO2@PEI. The hollow Ru-SiO2@PEI nanoparticles were used to build an ECL immunosensor for the analysis of neuron specific enolase (NSE). PEI not only assembled on the surface of Ru-SiO2 nanoparticles through the electrostatic interaction to act as co-reactant for Ru(bpy)32+ ECL, but also provided alkaline condition to etch the Ru-SiO2 nanoparticles to form the hollow Ru-SiO2@PEI nanoparticles with porous shell. The unique structure of the Ru-SiO2@PEI nanoparticles loaded both a large amount of Ru(bpy)32+ and its co-reactant PEI at the same time, which shortened the electron-transfer distance, thereby greatly enhanced the luminous efficiency and amplified the ECL signal. The developed immunosensor showed a wide linear range from 1.0 × 10−11 to 1.0 × 10−5 mg mL−1 with a low detection limit of 1.0 × 10−11 mg mL−1 for NSE. When the immunosensor was used for the determination of NSE in clinical human serum, the results were comparable with those obtained by using enzyme-linked immunosorbent assay (ELISA) method. The proposed method provides a promising alternative for NSE analysis in clinical samples.

Source: Nature.com

Bial's Fatal Clinical Trial in France Had Already Caused Deaths of Dogs in Earlier Tests

PARIS – An experimental chronic pain treatment that caused one death and left five others with possible brain damage in a French clinical lab was believed to be related to deaths in dogs in earlier clinical testing, the Telegraph of London reported this morning.

The Telegraph reported the details of the canine deaths were not reported by the laboratory conducting the tests for Portugal-based Bial-Portela were not disclosed due to “industrial secrecy.” The trial was being conducted by Bial, a clinical research organization in Rennes, France. Citing a report in the French paper Le Figaro, the Telegraph said a “number of dogs” died and others suffered neurological damage in preclinical trials of the drug. Bial has refused to reveal information about the preclinical data and the canine deaths.

Source: biospace.com

Bacteria take 'RNA mug shots' of threatening viruses

Scientists from The University of Texas at Austin, the Stanford University School of Medicine and two other institutions have discovered that bacteria have a system that can recognize and disrupt dangerous viruses using a newly identified mechanism involving ribonucleic acid (RNA). It is similar to the CRISPR/Cas system that captures foreign DNA. The discovery might lead to better ways to thwart viruses that kill agricultural crops and interfere with the production of dairy products such as cheese and yogurt.

Source: Sciencedaily.com

This is collected by Creative BioMart.

Creative BioMart Provides Quality and Comprehensive Assay Kits

An assay is an investigative method in laboratory medicine, pharmacology, natural science, and in addition molecular biology for subjectively evaluating the vicinity or measure of an objective substance. Assay kits, utilized as a part of life science research, drug disclosure and advancement, and ecological observing, have an extensive variety of utilization, for example, considering sickness pathways, screening for potential medication applicants and assessing biopharmaceutical production processes.

Since its inception, Creative BioMart has assisted scientists in the design and production of thousands of assay kits, with many resulting in high-impact citations. Recognized for its high quality standards and technical service, Creative BioMart has the knowledge and expertise to offer optimal assay kits to its customers.

Up to now, there are over 1670 assay kits offered in Creative BioMart including: U2OS C3AR1 Bioassay Kit, eXpress C5AR1 Activated GPCR Internalization Assay, CAMKK2 Assay Kit, cAMP CHO-K1 SSTR2 Bioassay Kit, CSF3R-JAK1 Bioassay Kit, Phosphate Assay Kit (Fluorometric), Fixable Dead Cell Staining Kit (Green Fluorescence 405), PKC kinase activity Kit, etc.

With assay kits in Creative BioMart, customers will receive all necessary reagents, plates, instructions, and analysis tools to help them find answers quickly and efficiently. We truly understand that our customers’ research is being done with finite time and money, so we create and refine our kits to be affordable and to provide fast, accurate results. Bulk assays and reagent sales are available upon your request. Please visit http://www.creativebiomart.net/product/assay-kits_358.htm for more information or just contact at info@creative-biomart.com & call 1-631-559-9269.

About Creative BioMart
Creative BioMart provides quality recombinant proteins, diagnostic antibodies and antigens, diagnostic enzymes and pharmaceutical enzymes to the research community of biology, clinical research, molecular diagnostics and biopharmaceutical drug development. We are offering more than 1,000 recombinant proteins, peptides and antibodies. All the products are rigorously tested to meet the most demanding research needs. At the same time, lowest prices in the industry are always guaranteed.

Contact Creative BioMart
Address: 45-16 Ramsey Road Shirley, NY 11967, USA
Tel: 1-631-559-9269
Email: info@creative-biomart.com
Website: www.creativebiomart.net

Cell Line Development and Membrane Preparation in Creative BioMart

A cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space. High quality cells and membranes supply is the key factor in GPCR & ion channel screening/profiling. 

With accumulated experience in the biotech field, Creative BioMart utilizes a proprietary technology to enable the expression of membrane proteins in mammalian cells at over a million molecules per cell surface. Supported by proprietary codon optimization and vector design technologies, scientists in Creative BioMart has developed a large product portfolio that covering almost 200 GRCR and ion channel stable cell lines. Moreover, to meet its customers’ different needs for the assay reagent products, membrane preparation and ready-to-use frozen cells products made from those stable cell lines are also available in Creative BioMart.

To be specific, Creative BioMart mainly serves the following four services for its Cell line development and membrane preparation service:

Stable cell line service for assay development
Stable cell line development is one of the important steps to develop a cell-based assay development. The company can deliver cell line of interest with high quality at fast turnaround time.

GPCR & Ion Channel Stable Cell Lines
The company provides a large collection of GPCR and ion channel stable cell lines for compound screening/profiling services. Furthermore, the customers can buy the stable cell lines and perform a screening project in-house.

GPCR & Ion Channel Membrane Preparation
With the application of high-expression GRCR & ion channel stable cell lines, Creative BioMart offers cell membrane preparations validated by receptor binding assays. Custom membrane preparation services are also available in Creative BioMart.

Frozen Cell Production 
It’s known that cell culturing is often challenging and time-consuming. Based on its state-of-the-art cell culture facilities, Creative BioMart’s cell culture service can quickly deliver the frozen cells that satisfy the needs of its customers’ individual project on time.

Key features of the service
Large cell line collection
Reliability
Convenience
Variability arrest

For more information about the membrane preparation service in Creative BioMart, please contact 1-631-559-9269.

Do you know biomarker?

Biomarker refers to the biochemical indicators that marking the changes of structure or function in systems, organs, tissues, cells and subcellular. It can be used for disease diagnosis, determining the stage of disease or to evaluate the safety and efficacy of new drugs or new therapies in the target population.

For disease research, biomarker generally refers to a biochemical indicator for objective measurement and evaluation of certain characteristics of general physiological or pathological or therapeutic procedure. The biological process of the body can be measured through biomarker testing. Checking of a disease-specific biomarkers may be helpful for the identification of disease, early diagnosis, prevention and treatment, and monitoring in the process of treatment. The search of valuable biomarkers has become an important focus of current research.

Classification

Biomarker of exposure

Biomarker of effect

Biomarker of susceptibility

Select principle

1. The selected biomarkers must have a certain specificity;

2. The selected biomarkers must have sufficient sensitivity;

3. Repeatability and individual differences analyzed by the selected biomarkers must within the acceptable range;

4. The selected biomarker must have sufficient stability for the transport, save and analysis of the sample;

Characteristics

1. Have certain sensitivity; higher than the general biological detection index;

2. Reactions have time effects; Reaction must have a certain setting time but fast;

3. The effect of the biomarker in the molecular and biochemical level must be closely related to the effect on the senior biology level (such as growth, reproduction); effect on all levels must have a causal relationship;

4. Have certain field value;

5. Specificity and early warning (eg. AchE)

Applications significance

Clarify the mechanism of various pollutants, determining the interaction between various pollutants and organisms; Combine with ecological effects to develop research programs to address environmental issues (such as population level drop, etc.).