Cell Line Development and Membrane Preparation in Creative BioMart

A cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space. High quality cells and membranes supply is the key factor in GPCR & ion channel screening/profiling. 

With accumulated experience in the biotech field, Creative BioMart utilizes a proprietary technology to enable the expression of membrane proteins in mammalian cells at over a million molecules per cell surface. Supported by proprietary codon optimization and vector design technologies, scientists in Creative BioMart has developed a large product portfolio that covering almost 200 GRCR and ion channel stable cell lines. Moreover, to meet its customers’ different needs for the assay reagent products, membrane preparation and ready-to-use frozen cells products made from those stable cell lines are also available in Creative BioMart.

To be specific, Creative BioMart mainly serves the following four services for its Cell line development and membrane preparation service:

Stable cell line service for assay development
Stable cell line development is one of the important steps to develop a cell-based assay development. The company can deliver cell line of interest with high quality at fast turnaround time.

GPCR & Ion Channel Stable Cell Lines
The company provides a large collection of GPCR and ion channel stable cell lines for compound screening/profiling services. Furthermore, the customers can buy the stable cell lines and perform a screening project in-house.

GPCR & Ion Channel Membrane Preparation
With the application of high-expression GRCR & ion channel stable cell lines, Creative BioMart offers cell membrane preparations validated by receptor binding assays. Custom membrane preparation services are also available in Creative BioMart.

Frozen Cell Production 
It’s known that cell culturing is often challenging and time-consuming. Based on its state-of-the-art cell culture facilities, Creative BioMart’s cell culture service can quickly deliver the frozen cells that satisfy the needs of its customers’ individual project on time.

Key features of the service
Large cell line collection
Reliability
Convenience
Variability arrest

For more information about the membrane preparation service in Creative BioMart, please contact 1-631-559-9269.

Do you know biomarker?

Biomarker refers to the biochemical indicators that marking the changes of structure or function in systems, organs, tissues, cells and subcellular. It can be used for disease diagnosis, determining the stage of disease or to evaluate the safety and efficacy of new drugs or new therapies in the target population.

For disease research, biomarker generally refers to a biochemical indicator for objective measurement and evaluation of certain characteristics of general physiological or pathological or therapeutic procedure. The biological process of the body can be measured through biomarker testing. Checking of a disease-specific biomarkers may be helpful for the identification of disease, early diagnosis, prevention and treatment, and monitoring in the process of treatment. The search of valuable biomarkers has become an important focus of current research.

Classification

Biomarker of exposure

Biomarker of effect

Biomarker of susceptibility

Select principle

1. The selected biomarkers must have a certain specificity;

2. The selected biomarkers must have sufficient sensitivity;

3. Repeatability and individual differences analyzed by the selected biomarkers must within the acceptable range;

4. The selected biomarker must have sufficient stability for the transport, save and analysis of the sample;

Characteristics

1. Have certain sensitivity; higher than the general biological detection index;

2. Reactions have time effects; Reaction must have a certain setting time but fast;

3. The effect of the biomarker in the molecular and biochemical level must be closely related to the effect on the senior biology level (such as growth, reproduction); effect on all levels must have a causal relationship;

4. Have certain field value;

5. Specificity and early warning (eg. AchE)

Applications significance

Clarify the mechanism of various pollutants, determining the interaction between various pollutants and organisms; Combine with ecological effects to develop research programs to address environmental issues (such as population level drop, etc.).